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Identification of a cytoplasmic complex that adds a cap onto 5'-monophosphate RNA.
Otsuka Y, Kedersha NL, Schoenberg DR
Mol Cell Biol. 2009 Apr;29(8):2155-67. doi: 10.1128/MCB.01325-08. Epub 2009 Feb 17.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
82459pcDNA3-myc-mCEExpression of myc epitope and murine mRNA Capping Enzyme in mammalian cells
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82461pcDNA3-myc-mCE(K294A)Expression of myc epitope and catalytic inactive form of murine mRNA Capping Enzyme (K294A) in mammalian cells
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82462pcDNA3-myc-mCE(211-597)Expression of myc epitope and C-terminal domain of murine mRNA Capping Enzyme in mammalian cells
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82463pcDNA3-myc NES-mCEExpression of myc epitope and murine mRNA Capping Enzyme with Terminal nuclear export signal in mammalian cells
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82464pcDNA3-myc NES- mCE(K294A)Expresses myc tag and catalytic inactive form of mRNA capping enzyme with N terminal Nuclear export signal (NES)
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82465pcDNA3-myc-mCE NLSExpresses myc tag and mRNA capping enzyme without Nuclear Localization Sequence (NLS) from C terminus
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82466pcDNA3-myc NES-mCE NLSExpresses cytoplasmic restricted form of myc tagged mRNA capping enzyme
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82467pcDNA3- myc NES-mCE(K294A) NLSExpresses cytoplasmic restricted form of myc tagged mRNA capping enzyme
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82468pcDNA4/TO-myc NES-mCE NLS-FlagExpresses cytoplasmic restricted form of N-terminal myc tagged and C-terminal flag tagged mRNA capping enzyme
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82469pcDNA4/TO-myc NES-mCE(K294A) NLS-FlagExpresses cytoplasmic restricted form of N-terminal myc tagged and C-terminal flag tagged mRNA capping enzyme, inactive form
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82470pcDNA4/TO-myc-mCEExpression of myc epitope and murine mRNA Capping Enzyme in mammalian cells
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82471pcDNA4/TO-myc- GFP-FlagExpresses myc-GFP-Flag
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Antibodies from Article