pQE30-roGFP1-iL
(Plasmid #83313)

• Purpose: Inducible expression of redox sensitive GFP in bacteria
• Depositing Lab: S. James Remington
• Publication: Lohman et al Biochemistry. 2008 Aug 19;47(33):8678-88. doi: 10.1021/bi800498g. Epub 2008 Jul 25.

Backbone

• Vector backbone: pQE30
• Backbone manufacturer: Qiagen
• Backbone size w/o insert (bp): 3400
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: roGFP1-iL
• Alt name: ro1/iL
• Species: jellyfish
• Insert Size (bp): 780
• Mutation: ro1(C48S/S147C/Q204C) plus X insertion, iL between 147C and H148S/F64L/F99S/M153T/V163A/I167T
• Promoter: T5
• Tag / Fusion Protein:
  • 6xHIS (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: pQE-F2
• 3′ sequencing primer: T0term-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry