pRSETB-roGFP1-R12
(Plasmid
#83311)
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PurposeInducible expression of redox sensitive GFP in bacteria
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 83311 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepRSETB
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 2900
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameroGFP1-R12
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Alt namero1/12
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Speciesjellyfish
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Insert Size (bp)714
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MutationC48S/Q80R/S147C/Q204C/N149K/F223R/S202K
- Promoter T7
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Tag
/ Fusion Protein
- 6xHIS (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7 terminal (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRSETB-roGFP1-R12 was a gift from S. James Remington (Addgene plasmid # 83311 ; http://n2t.net/addgene:83311 ; RRID:Addgene_83311) -
For your References section:
Re-engineering redox-sensitive green fluorescent protein for improved response rate. Cannon MB, Remington SJ. Protein Sci. 2006 Jan;15(1):45-57. Epub 2005 Dec 1. 10.1110/ps.051734306 PubMed 16322566