Purposefull length human gamma ENaC with myc tag
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||83428||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namehuman gamma ENaC
SpeciesH. sapiens (human)
Insert Size (bp)2045
- Promoter CMV
/ Fusion Protein
- Myc (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Terms and Licenses
Please note the ENaC insert in this plasmid contains a single nucleotide mismatch compared to Genbank reference sequence AAA75460.1, which causes a P502A mutation of unknown significance. The ENaC sequence containing 502A was cloned and reported in McDonald et al., Am J Physiol. 1995 May; 268(5 Pt 1): C1157-63. PMID: 7762608 (see Figure 1B) and was the source of the ENaC insert for this plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:h-gamma-ENaC Myc was a gift from Christie Thomas (Addgene plasmid # 83428 ; http://n2t.net/addgene:83428 ; RRID:Addgene_83428)
For your References section:Nedd4-2 isoforms ubiquitinate individual epithelial sodium channel subunits and reduce surface expression and function of the epithelial sodium channel. Raikwar NS, Thomas CP. Am J Physiol Renal Physiol. 2008 May;294(5):F1157-65. doi: 10.1152/ajprenal.00339.2007. Epub 2008 Mar 5. 10.1152/ajprenal.00339.2007 PubMed 18322022