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h-gamma-ENaC Myc
(Plasmid #83428)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 83428 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human gamma ENaC
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2045
  • Mutation
    P502A
  • Promoter CMV
  • Tag / Fusion Protein
    • Myc (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

Please note the ENaC insert in this plasmid contains a single nucleotide mismatch compared to Genbank reference sequence AAA75460.1, which causes a P502A mutation of unknown significance. The ENaC sequence containing 502A was cloned and reported in McDonald et al., Am J Physiol. 1995 May; 268(5 Pt 1): C1157-63. PMID: 7762608 (see Figure 1B) and was the source of the ENaC insert for this plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    h-gamma-ENaC Myc was a gift from Christie Thomas (Addgene plasmid # 83428 ; http://n2t.net/addgene:83428 ; RRID:Addgene_83428)
  • For your References section:

    Nedd4-2 isoforms ubiquitinate individual epithelial sodium channel subunits and reduce surface expression and function of the epithelial sodium channel. Raikwar NS, Thomas CP. Am J Physiol Renal Physiol. 2008 May;294(5):F1157-65. doi: 10.1152/ajprenal.00339.2007. Epub 2008 Mar 5. 10.1152/ajprenal.00339.2007 PubMed 18322022