h-alpha-ENaC-2
(Plasmid #83431)

• Purpose: full length human alpha ENaC-2 with flag tag
• Depositing Lab: Christie Thomas
• Publication: Thomas et al Am J Physiol. 1998 May;274(5 Pt 1):C1312-23.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: thermofisher
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: human alpha ENaC isoform 2
• Species: H. sapiens (human)
• Insert Size (bp): 2190
• Entrez Gene: SCNN1A (a.k.a. BESC2, ENaCa, ENaCalpha, LIDLS3, PHA1B1, SCNEA, SCNN1)
• Promoter: CMV
• Tag / Fusion Protein:
  • Flag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Supplemental Documents:
  • Genbank file of plasmid

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry