PurposeExpresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains NatMX3 marker for yeast transformation.
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||83477||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeYeast Expression
Selectable markersnourseothricin (nat)
Growth in Bacteria
Growth Strain(s)NEB dam-/dcm-
Growth instructionsGrowth in dam- E.coli cells is preferred
Copy numberHigh Copy
- Cloning method Gibson Cloning
- 5′ sequencing primer pRS-marker
- 3′ sequencing primer F1ori-F (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byAddgene plasmid #67638 pML104, deposited by John Wyrick
Terms and Licenses
- Not Available to Industry
This plasmid is similar to pML104 except that the URA3 marker has been switched for NatMx3 marker using Gibson Assembly. Cloning of guide sequence using BclI-SwaII restriction sites requires growth in dam- E. coli cells.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pML104-NatMx3 was a gift from Patricia Wittkopp (Addgene plasmid # 83477 ; http://n2t.net/addgene:83477 ; RRID:Addgene_83477)