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Patricia Wittkopp Lab: Patricia Wittkopp yeast CRISPR plasmids
Unpublished

Plasmids from Article

ID Plasmid Purpose  
83475pML104-HygMx4Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains HphMx4 marker for yeast transformation.
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83476pML104-KanMx4Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains KanMx4 marker for yeast transformation.
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83477pML104-NatMx3Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains NatMX3 marker for yeast transformation.
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