pcDL-hu FceRI alpha
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||8365||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepcDL SR alpha 296
Backbone manufacturerK Arai DNAX
- Backbone size w/o insert (bp) 3741
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namehuman Fc epsilon RI alpha cDNA
Alt namehigh affinity receptor for IgE alpha chain
SpeciesH. sapiens (human)
Insert Size (bp)1177
Entrez GeneFCER1A (a.k.a. FCE1A, FcERI)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer GTT GCC TTT ACT TCT AGG CCT
- 3′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
For information about the empty vector, see Yokota et al - PNAS Nov 1987. Vol 84, p 7388-7392.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDL-hu FceRI alpha was a gift from Jean Kinet (Addgene plasmid # 8365 ; http://n2t.net/addgene:8365 ; RRID:Addgene_8365)
For your References section:Isolation of the gene coding for the alpha subunit of the human high affinity IgE receptor. Kochan J, Pettine LF, Hakimi J, Kishi K, Kinet JP. Nucleic Acids Res 1988 Apr 25;16(8):3584. 10.1093/nar/16.8.3584 PubMed 2967464