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pcDL-hu FceRI alpha
(Plasmid #8365)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 8365 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDL SR alpha 296
  • Backbone manufacturer
    K Arai DNAX
  • Backbone size w/o insert (bp) 3741
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human Fc epsilon RI alpha cDNA
  • Alt name
    high affinity receptor for IgE alpha chain
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1177
  • GenBank ID
    X06948
  • Entrez Gene
    FCER1A (a.k.a. FCE1A, FcERI)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer GTT GCC TTT ACT TCT AGG CCT
  • 3′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

For information about the empty vector, see Yokota et al - PNAS Nov 1987. Vol 84, p 7388-7392.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDL-hu FceRI alpha was a gift from Jean Kinet (Addgene plasmid # 8365 ; http://n2t.net/addgene:8365 ; RRID:Addgene_8365)
  • For your References section:

    Isolation of the gene coding for the alpha subunit of the human high affinity IgE receptor. Kochan J, Pettine LF, Hakimi J, Kishi K, Kinet JP. Nucleic Acids Res 1988 Apr 25;16(8):3584. 10.1093/nar/16.8.3584 PubMed 2967464