pBJ1 neo-hu FceRI gamma
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16540||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepBJ1 neo
Backbone manufacturerMark M Davis
- Backbone size w/o insert (bp) 6600
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Alt namehuman FcR gamma chain
SpeciesH. sapiens (human)
Insert Size (bp)421
Entrez GeneFCER1G (a.k.a. FCRG)
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site NotI (destroyed during cloning)
- 5′ sequencing primer n/a
- 3′ sequencing primer n/a (Common Sequencing Primers)
This construct contains only the coding sequence. It was first used in Lin S, Cicala C, Scharenberg AM, Kinet J-P (1996) Cell 85, 985-995. It can be sequenced with primers within the insert.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBJ1 neo-hu FceRI gamma was a gift from Jean Kinet (Addgene plasmid # 16540 ; http://n2t.net/addgene:16540 ; RRID:Addgene_16540)
For your References section:Characterization and expression of the gene for the human Fc receptor gamma subunit. Definition of a new gene family. Kuster H, Thompson H, Kinet JP. J Biol Chem 1990 Apr 15;265(11):6448-52. PubMed 2138619