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pGEM-human TCR zeta/2470
(Plasmid #11507)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 11507 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGEM-3
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 3200
  • Vector type
    general cloning

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TCR zeta
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1700
  • Mutation
    n/a
  • GenBank ID
    J04132
  • Entrez Gene
    CD247 (a.k.a. CD3-ZETA, CD3H, CD3Q, CD3Z, IMD25, T3Z, TCRZ)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xba I (not destroyed)
  • 3′ cloning site EcoR I (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEM-human TCR zeta/2470 was a gift from Allan Weissman (Addgene plasmid # 11507 ; http://n2t.net/addgene:11507 ; RRID:Addgene_11507)
  • For your References section:

    Molecular cloning and chromosomal localization of the human T-cell receptor zeta chain: distinction from the molecular CD3 complex. Weissman AM, Hou D, Orloff DG, Modi WS, Seuanez H, O'Brien SJ, Klausner RD. Proc Natl Acad Sci U S A. 1988 Dec . 85(24):9709-13. 10.1073/pnas.85.24.9709 PubMed 2974162