PurposeMammalian Display vector
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||60606||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5428
- Total vector size (bp) 7272
Vector typeMammalian Expression
Growth in Bacteria
Growth Strain(s)NEB Stable
Gene/Insert namePS11-scFvFc-CD28-gp41 (706-713)
SpeciesH. sapiens (human)
Insert Size (bp)1844
- Promoter CMV
/ Fusion Protein
- C9 (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site AscI (not destroyed)
- 3′ cloning site PacI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer BGHRev (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3.1-PS11-scFvFc-CD28-gp41 (706-713) was a gift from Wayne Marasco (Addgene plasmid # 60606 ; http://n2t.net/addgene:60606 ; RRID:Addgene_60606)
For your References section:Lentivirus display: stable expression of human antibodies on the surface of human cells and virus particles. Taube R, Zhu Q, Xu C, Diaz-Griffero F, Sui J, Kamau E, Dwyer M, Aird D, Marasco WA. PLoS One. 2008 Sep 11;3(9):e3181. doi: 10.1371/journal.pone.0003181. 10.1371/journal.pone.0003181 PubMed 18784843
Map uploaded by the depositor.