PurposeMammalian expression of mouse GATA3
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||83816||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Backbone manufacturerGorman, et al. 1982
Modifications to backboneCAT was removed
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
SpeciesM. musculus (mouse)
Entrez GeneGata3 (a.k.a. Gata-3, jal)
- Promoter RSV
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer pREP-F (GCTCGATACAATAAACGCC)
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:RSV/mGATA3 (sense) was a gift from Douglas Engel (Addgene plasmid # 83816 ; http://n2t.net/addgene:83816 ; RRID:Addgene_83816)
For your References section:Murine and human T-lymphocyte GATA-3 factors mediate transcription through a cis-regulatory element within the human T-cell receptor delta gene enhancer. Ko LJ, Yamamoto M, Leonard MW, George KM, Ting P, Engel JD. Mol Cell Biol. 1991 May . 11(5):2778-84. PubMed 2017177