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pET30-2-PSPH
(Plasmid #83909)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 83909 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET30-2
  • Backbone size w/o insert (bp) 5647
  • Total vector size (bp) 6352
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    phosphoserine phosphatase
  • Alt name
    PSPH
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    705
  • GenBank ID
    NM_004577.3
  • Entrez Gene
    PSPH (a.k.a. PSP, PSPHD)
  • Promoter T7
  • Tag / Fusion Protein
    • 6x His tags (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer T7 Promoter
  • 3′ sequencing primer T7 Terminator
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET30-2-PSPH was a gift from David Sabatini (Addgene plasmid # 83909 ; http://n2t.net/addgene:83909 ; RRID:Addgene_83909)
  • For your References section:

    A PHGDH inhibitor reveals coordination of serine synthesis and one-carbon unit fate. Pacold ME, Brimacombe KR, Chan SH, Rohde JM, Lewis CA, Swier LJ, Possemato R, Chen WW, Sullivan LB, Fiske BP, Cho S, Freinkman E, Birsoy K, Abu-Remaileh M, Shaul YD, Liu CM, Zhou M, Koh MJ, Chung H, Davidson SM, Luengo A, Wang AQ, Xu X, Yasgar A, Liu L, Rai G, Westover KD, Vander Heiden MG, Shen M, Gray NS, Boxer MB, Sabatini DM. Nat Chem Biol. 2016 Jun;12(6):452-8. doi: 10.1038/nchembio.2070. Epub 2016 Apr 25. 10.1038/nchembio.2070 PubMed 27110680