Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||8406||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 7500
Vector typeMammalian Expression, Cre/Lox
Growth in Bacteria
Copy numberHigh Copy
Alt namemodified Cre/lox
- Cloning method Restriction Enzyme
- 5′ cloning site DraIII (not destroyed)
- 3′ cloning site EcoR47III (destroyed during cloning)
- 5′ sequencing primer na (Common Sequencing Primers)
DraIII and EcoICRI fragment containing the CMV-STOP-luc cassette from p231 pCMVe-betaAc-STOP luc ligated to the DraIII and Eco47III fragment from p236 pPr-CREM containing probasin-modified cre.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:p252 pPr-CREM/CMV-STOP-luc was a gift from Jeffrey Green (Addgene plasmid # 8406 ; http://n2t.net/addgene:8406 ; RRID:Addgene_8406)
For your References section:A single vector containing modified cre recombinase and LOX recombination sequences for inducible tissue-specific amplification of gene expression. Kaczmarczyk SJ, Green JE. Nucleic Acids Res 2001 Jun 15;29(12):E56-6. 10.1093/nar/29.12.e56 PubMed 11410679