Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||8408||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 7500
Vector typeMammalian Expression, Cre/Lox
Growth in Bacteria
Copy numberHigh Copy
Alt namemodified Cre/lox
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRICI (destroyed during cloning)
- 3′ cloning site DraIII (destroyed during cloning)
- 5′ sequencing primer na (Common Sequencing Primers)
Chicken globin insulators between Pr-CREM and CMV-STOP-luc cassettes. Pr-CREM from p240 pPr-CREM/Ins isolated with DraIII and Eco47III and ligated into EcoRICI/DraIII fragment of pCMVe-betaAc-STOP-luc (p231).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:p255 pPr-CREM/Ins/CMV-STOP-luc was a gift from Jeffrey Green (Addgene plasmid # 8408 ; http://n2t.net/addgene:8408 ; RRID:Addgene_8408)
For your References section:A single vector containing modified cre recombinase and LOX recombination sequences for inducible tissue-specific amplification of gene expression. Kaczmarczyk SJ, Green JE. Nucleic Acids Res 2001 Jun 15;29(12):E56-6. 10.1093/nar/29.12.e56 PubMed 11410679
Map uploaded by the depositor.