pMycoFos
(Plasmid #84577)

• Purpose: (Empty Backbone) Shuttle and expression vector. Replicates in E.coli and Mycobacterium. Gives acetamide-inducible gene expression in Mycobacterium. Copy number is controllable by arabinose in E.coli.
• Depositing Lab: Nicholas Coleman
• Publication: Ly et al J Microbiol Methods. 2011 Sep;86(3):320-6. doi: 10.1016/j.mimet.2011.06.005. Epub 2011 Jun 13.

Backbone

• Vector backbone: pMycoFos
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): EPI300
• Growth instructions: Sample requires arabinose for induction.
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: None

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

For maintenance of vector, any standard E.coli is OK, but to make plasmid preps, the plasmid must be propagated in EPI300 or similar strain that enables the arabinose-inducible copy control system. Induction of gene expression is with acetamide in Mycobacterium, but this doesnt work in E.coli. Some level of control over expression in E.coli can be gained via controlling the copy number of the plasmid with arabinose. See published paper for more details on construction and use of pMycoFos. Has unique EcoRI, PacI, NheI, SwaI, BamHI sites for cloning