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pcDNA3-mCherry-LKB1(KD)
(Plasmid #84640)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 84640 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5500
  • Total vector size (bp) 7509
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mCherry-LBK1(K78I)
  • Alt name
    LKB1 (KD)
  • Alt name
    STK11
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    2022
  • Mutation
    Lysine 78 mutated to Isoleucine; kinase dead
  • GenBank ID
    NC_000019.10
  • Entrez Gene
    STK11 (a.k.a. LKB1, PJS, hLKB1)
  • Promoter CMV
  • Tags / Fusion Proteins
    • mCherry (N terminal on insert)
    • 6xHis (N terminal on backbone)
    • T7 tag (gene 10 leader) (N terminal on backbone)
    • Xpress (TM) tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer T7 forward
  • 3′ sequencing primer BGH reverse
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-mCherry-LKB1(KD) was a gift from Jin Zhang (Addgene plasmid # 84640 ; http://n2t.net/addgene:84640 ; RRID:Addgene_84640)
  • For your References section:

    Visualization of Compartmentalized Kinase Activity Dynamics Using Adaptable BimKARs. Depry C, Mehta S, Li R, Zhang J. Chem Biol. 2015 Nov 19;22(11):1470-9. doi: 10.1016/j.chembiol.2015.10.004. Epub 2015 Nov 5. 10.1016/j.chembiol.2015.10.004 PubMed 26548610