|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||84652||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Modifications to backboneinsert gateway cassette
Vector typeBacterial Expression
- Promoter T7
/ Fusion Proteins
- Trx Tag (N terminal on backbone)
- His (N terminal on backbone)
- S tag (N terminal on backbone)
- His (C terminal on backbone)
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol and Ampicillin
Growth Strain(s)ccdB Survival
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pET32a-DEST was a gift from Neil Olszewski (Addgene plasmid # 84652 ; http://n2t.net/addgene:84652 ; RRID:Addgene_84652)