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pKSCTMF
(Plasmid #85073)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 85073 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBluescript II KS(-)
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 2900
  • Total vector size (bp) 6000
  • Vector type
    Gene insertion/deletion in Pichia pastoris
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AOX1promoter - mazF - AOX1 tt- Zeocin R gene
  • Species
    E. coli
  • Insert Size (bp)
    3000
  • GenBank ID
    mazF NP_417262.1
  • Promoter pAOX1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRV (unknown if destroyed)
  • 3′ cloning site SmaI (unknown if destroyed)
  • 5′ sequencing primer T7 and 5 AOX1
  • 3′ sequencing primer T3 and 3AOX1
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    pDGIEF _Prof. Shun-Peng Li lab, Nanjing Agricultural University
  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKSCTMF was a gift from Sheng Yang (Addgene plasmid # 85073 ; http://n2t.net/addgene:85073 ; RRID:Addgene_85073)
  • For your References section:

    mazF as a counter-selectable marker for unmarked genetic modification of Pichia pastoris. Yang J, Jiang W, Yang S. FEMS Yeast Res. 2009 Jun;9(4):600-9. doi: 10.1111/j.1567-1364.2009.00503.x. Epub 2009 Mar 25. 10.1111/j.1567-1364.2009.00503.x PubMed 19416369