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hCdh1 (1-125)
(Plasmid #8508)

Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 8508 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 5169
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    hCdh1 (1-125)
  • Alt name
    hCdh1 N terminus
  • Alt name
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Mutation
    Cdh1 N-terminus. Dominant Negative mutant.
  • Entrez Gene
    FZR1 (a.k.a. CDC20C, CDH1, DEE109, FZR, FZR2, HCDH, HCDH1)
  • Tag / Fusion Protein
    • His (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer pBABE 5'
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See author's map. For production of retrovirus expressing human Cdh1 N-terminus. (Ruderman RC230)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hCdh1 (1-125) was a gift from Joan Ruderman (Addgene plasmid # 8508 ; ; RRID:Addgene_8508)
  • For your References section:

    Requirements for the destruction of human Aurora-A. Crane R, Kloepfer A, Ruderman JV. J Cell Sci 2004 Dec 1;117(Pt 25):5975-83. Epub 2004 Nov 9. 10.1242/jcs.01418 PubMed 15536123