HOXB3 (human) FLAG pSP65
(Plasmid #8523)

• Depositing Lab: Corey Largman
• Publication: Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-906.

Backbone

• Vector backbone: pSP65
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 3005
• Vector type: Bacterial Expression ; In vitro transcription/translation

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HOXB3
• Alt name: HOX2G
• Alt name: HOX2.7
• Species: H. sapiens (human)
• Insert Size (bp): 1644
• Mutation: Contains 5 additional amino acids before ATG from cloning vectors; makes protein by Western
• GenBank ID: X16667
• Entrez Gene: HOXB3 (a.k.a. HOX2, HOX2G, Hox-2.7)
• Tag / Fusion Protein:
  • FLAG (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: KpnI in Bsk linker (not destroyed)
• 5′ sequencing primer: SP6

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Guy Sauvageau

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

used to make protein in vitro