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Addgene

HOXB5 (human) FLAG-MSCV
(Plasmid #8532)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 8532 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    MSCV
  • Backbone size w/o insert (bp) 6400
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    GFP for FACS sorting

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HOXB5
  • Alt name
    HOX2A
  • Alt name
    HOX2.1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    848
  • Mutation
    contains improved ribosomal binding site and FLAG epitope tag on N-terminal, starts at AA; #2, makes protein by western; contains GFP driven from an IRES
  • Entrez Gene
    HOXB5 (a.k.a. HHO.C10, HOX2, HOX2A, HU-1, Hox2.1)
  • Tags / Fusion Proteins
    • FLAG (N terminal on insert)
    • IRES-GFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer na
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Craig Hauser

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See author's map.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HOXB5 (human) FLAG-MSCV was a gift from Corey Largman (Addgene plasmid # 8532 ; http://n2t.net/addgene:8532 ; RRID:Addgene_8532)
  • For your References section:

    Lineage-restricted expression of homeobox-containing genes in human hematopoietic cell lines. Shen WF, Largman C, Lowney P, Corral JC, Detmer K, Hauser CA, Simonitch TA, Hack FM, Lawrence HJ. Proc Natl Acad Sci U S A 1989 Nov;86(21):8536-40. 10.1073/pnas.86.21.8536 PubMed 2573064