gRNA Cloning Vector Bbs I ver. 2
Purpose(Empty Backbone) An empty sgRNA expression vector. sgRNA sequence can be cloned into its Bbs I site. Its gRNA scaffold has efficient ver. 2 structure.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||85586||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Backbone manufacturerLife Technologies
- Backbone size (bp) 2380
Vector typeMammalian Expression, CRISPR
- Promoter U6
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer M13 Forward, T7 (Common Sequencing Primers)
Articles Citing this Plasmid
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:gRNA Cloning Vector Bbs I ver. 2 was a gift from Hodaka Fujii (Addgene plasmid # 85586 ; http://n2t.net/addgene:85586 ; RRID:Addgene_85586)
For your References section:Allele-specific locus binding and genome editing by CRISPR at the p16INK4a locus. Fujita T, Yuno M, Fujii H. Sci Rep. 2016 Jul 28;6:30485. doi: 10.1038/srep30485. 10.1038/srep30485 PubMed 27465215