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gRNA Cloning Vector Bbs I ver. 2
(Plasmid #85586)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 85586 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pMA-T
  • Backbone manufacturer
    Life Technologies
  • Backbone size (bp) 2380
  • Vector type
    Mammalian Expression, CRISPR
  • Promoter U6

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    gRNA Cloning Vector Bbs I ver. 2 was a gift from Hodaka Fujii (Addgene plasmid # 85586 ; http://n2t.net/addgene:85586 ; RRID:Addgene_85586)
  • For your References section:

    Allele-specific locus binding and genome editing by CRISPR at the p16INK4a locus. Fujita T, Yuno M, Fujii H. Sci Rep. 2016 Jul 28;6:30485. doi: 10.1038/srep30485. 10.1038/srep30485 PubMed 27465215