pET28b-PU.1-ETS
(Plasmid #85734)

• Purpose: Expresses DNA-binding (ETS) domain of murine PU.1, residues 167-272
• Depositing Lab: Gregory Poon
• Publication: Wang et al J Biol Chem. 2014 Aug 1;289(31):21605-16. doi: 10.1074/jbc.M114.575340. Epub 2014 Jun 21.

Backbone

• Vector backbone: pET28b
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5246
• Total vector size (bp): 5612
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Use BL21*(DE3) for expression and induce at 30°C
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: ETS domain of murine PU.1 (residues 167-272)
• Alt name: Spi1
• Alt name: spleen focus forming virus (SFFV) proviral integration oncogene
• Species: H. sapiens (human), M. musculus (mouse)
• Insert Size (bp): 367
• GenBank ID: NM_011355.2 NP_035485.1
• Entrez Gene: Spi1 (a.k.a. Dis-1, Dis1, PU.1, Sfpi-1, Sfpi1, Spi-1, Tcfpu1, Tfpu.1)
• Tag / Fusion Protein:
  • 6xHis following thrombin cleavage site LVPR|G (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: T7 terminator

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry