pET28b-Ets1-ETS
(Plasmid #85735)

• Purpose: Expresses DNA-binding (ETS) domain of murine Ets-1, residues 311 to 440
• Depositing Lab: Gregory Poon
• Publication: Wang et al J Biol Chem. 2014 Aug 1;289(31):21605-16. doi: 10.1074/jbc.M114.575340. Epub 2014 Jun 21.

Backbone

• Vector backbone: pET28b
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 5231
• Total vector size (bp): 377
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Use BL21*(DE3) for expression and induce at 30°C
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: DNA-binding (ETS) domain of murine Ets-1, residues 331 to 440
• Alt name: E26 avian leukemia oncogene 1, 5' domain
• Species: H. sapiens (human), M. musculus (mouse)
• Insert Size (bp): 377
• GenBank ID: NP_035938.2
• Entrez Gene: Ets1 (a.k.a. D230050P06, Ets-1, Tpl1, p54, vs)
• Tag / Fusion Protein:
  • 6xHis following thrombin cleavage site LVPR|G (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: T7 terminator

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note that the DNA sequence in this plasmid is PCRed from the human gene; however, the human and mouse ETS domains are identical at the amino acid level.