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delNC C-His Ana GvpC in pET28a
(Plasmid #85736)


Item Catalog # Description Quantity Price (USD)
Plasmid 85736 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 5206
  • Total vector size (bp) 5737
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    Gas vesicle protein C (truncated and his-tagged)
  • Alt name
    delNC GvpC
  • Species
    Anabaena flos-aquae
  • Insert Size (bp)
  • Mutation
    Deletion of N and C terminal regions, addition of glycine after the start codon to keep the NcoI site without changing the translation frame, 3 amino acid linker between truncated GvpC and His-tag
  • Promoter T7
  • Tag / Fusion Protein
    • His-tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7 forward
  • 3′ sequencing primer T7 terminator reverse
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    delNC C-His Ana GvpC in pET28a was a gift from Mikhail Shapiro (Addgene plasmid # 85736 ; ; RRID:Addgene_85736)
  • For your References section:

    Molecular Engineering of Acoustic Protein Nanostructures. Lakshmanan A, Farhadi A, Nety SP, Lee-Gosselin A, Bourdeau RW, Maresca D, Shapiro MG. ACS Nano. 2016 Aug 23;10(8):7314-22. doi: 10.1021/acsnano.6b03364. Epub 2016 Jun 30. 10.1021/acsnano.6b03364 PubMed 27351374