pPBX1-318
(Plasmid
#8589)
-
Depositing Lab
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 8589 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
-
Vector backbonePET21b
-
Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5443
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberLow Copy
Gene/Insert
-
Gene/Insert nameHuman pbx (1-318)
-
Alt namePBX1
-
SpeciesH. sapiens (human)
-
Insert Size (bp)954
-
Entrez GenePBX1 (a.k.a. CAKUHED)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pPBX1-318 was a gift from Cynthia Wolberger (Addgene plasmid # 8589 ; http://n2t.net/addgene:8589 ; RRID:Addgene_8589) -
For your References section:
Structure of HoxA9 and Pbx1 bound to DNA: Hox hexapeptide and DNA recognition anterior to posterior. LaRonde-LeBlanc NA, Wolberger C. Genes Dev 2003 Aug 15;17(16):2060-72. 10.1101/gad.1103303 PubMed 12923056
