Purposehuman VEGFR1 with V to A mutation at position 767
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||86038||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector backbonepCMV6 Entry
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberHigh Copy
Gene/Insert namehuman Flt1 V767A
SpeciesH. sapiens (human)
Insert Size (bp)4286
Entrez GeneFLT1 (a.k.a. FLT, FLT-1, VEGFR-1, VEGFR1)
- Promoter CMV
/ Fusion Proteins
- FLAG (C terminal on backbone)
- HA (please see depositor comments below) (N terminal on insert)
- Myc (C terminal on backbone)
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer hGH-PA-R (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Please note: Since the signal sequence (the first few AA) for Flt1 is cleaved off , the HA tag was placed between AA30-31. The final mature protein has the HA tag N-terminal to the protein.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:hFlt1 V767A was a gift from Christie Thomas (Addgene plasmid # 86038 ; http://n2t.net/addgene:86038 ; RRID:Addgene_86038)
For your References section:Ectodomain cleavage of FLT1 regulates receptor activation and function and is not required for its downstream intracellular cleavage. Raikwar NS, Liu KZ, Thomas CP. Exp Cell Res. 2016 May 15;344(1):103-11. doi: 10.1016/j.yexcr.2016.03.020. Epub 2016 Mar 23. 10.1016/j.yexcr.2016.03.020 PubMed 27017929