pBJ1 neo-mo FceRI beta
(Plasmid
#8604)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 8604 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepBJ1 neo
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Backbone manufacturerMarc M Davis
- Backbone size w/o insert (bp) 6600
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemouse Fc epsilon RI beta cDNA
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Alt namehigh affinity receptor I for IgE beta chain
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Alt namemembrane-spanning 4-domains
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SpeciesM. musculus (mouse)
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Insert Size (bp)1010
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GenBank IDJ05019
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Entrez GeneMs4a2 (a.k.a. FcRB, Fce1b, Fcer1b, Fcrbeta, Ms4a1, fcERI)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer SV40pro-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBJ1 neo-mo FceRI beta was a gift from Jean Kinet (Addgene plasmid # 8604 ; http://n2t.net/addgene:8604 ; RRID:Addgene_8604) -
For your References section:
Complete structure of the mouse mast cell receptor for IgE (Fc epsilon RI) and surface expression of chimeric receptors (rat-mouse-human) on transfected cells. Ra C, Jouvin MH, Kinet JP. J Biol Chem 1989 Sep 15;264(26):15323-7. PubMed 2527850