sFlt1-e15a V5
(Plasmid
#86045)
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Purposehuman Soluble Flt1 isoform e15a (terminates in exon 15a)
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Depositing Lab
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Depositing OrganizationUniversity of Iowa
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 86045 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 86045-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 86045-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backboneTOPO Directional
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Backbone manufacturerthermofisher
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehuman soluble Flt1 isoform e15a
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SpeciesH. sapiens (human)
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Insert Size (bp)2342
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Mutationisoform e15a (terminates in exon 15a)
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Entrez GeneFLT1 (a.k.a. FLT, FLT-1, VEGFR-1, VEGFR1)
- Promoter CMV
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Tags
/ Fusion Proteins
- 6xHis (C terminal on backbone)
- V5 (C terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-Rev
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 86045-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 86045-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
sFlt1-e15a V5 was a gift from Christie Thomas (Addgene plasmid # 86045 ; http://n2t.net/addgene:86045 ; RRID:Addgene_86045) -
For your References section:
A recently evolved novel trophoblast-enriched secreted form of fms-like tyrosine kinase-1 variant is up-regulated in hypoxia and preeclampsia. Thomas CP, Andrews JI, Raikwar NS, Kelley EA, Herse F, Dechend R, Golos TG, Liu KZ. J Clin Endocrinol Metab. 2009 Jul;94(7):2524-30. doi: 10.1210/jc.2009-0017. Epub 2009 Mar 31. 10.1210/jc.2009-0017 PubMed 19336504
