pME-mCherry-24xMBS
(Plasmid #86242)

• Purpose: (Empty Backbone) Middle entry vector for generating MBS tagged RNAs with a fluorescent protein reporter
• Depositing Lab: Florence Marlow
• Publication: Campbell et al Development. 2015 Apr 1;142(7):1368-74. doi: 10.1242/dev.118968. Epub 2015 Mar 10.

Backbone

• Vector backbone: PCR8/GW/TOPO (K250020, Invitrogen)
• Backbone manufacturer: Invitrogen
• Modifications to backbone: pME-mCherry-24xMBS was created by amplifying mCherry with flanking BamHI sites by PCR from p3E-mCherry (Kwan et al., 2007) using p3E-mCherry-BamHI-F+R primers, then ligating into pME-24xMBS
• Vector type: Unspecified
• Tag / Fusion Protein:
  • mCherry-24xMBS (C terminal on insert)

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Resource Information

• Addgene Notes:
  • Addgene diagnostic digest

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note: due the the repetitive nature of the cassette, Addgene QC did not verify the presence of all 24x MBS; digest results indicate all sites are present based on size.