Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

pTol-Promoter:NLS-tdMCP-eGFP
(Plasmid #86248)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 86248 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pTolDestR4-R2pA
  • Backbone manufacturer
    Villefranc et al., 2007
  • Modifications to backbone
    The βactin (Higashijima et al., 1997) and hsp70l (Halloran et al., 2000) promoter 5′ entry clones (p5E) were previously described (Kwan et al., 2007). Appropriate p5E and pME plasmids were recombined with pTolDestR4-R2pA (Villefranc et al., 2007) following the manufacturer’s instructions (Invitrogen) to make pTolBetaActin: NLS-tdMCP-eGFP.
  • Vector type
    Used with transposase to make transient or stable transgenic zebrafish lines

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    beta Actin promoter:NLS-tdMCPeGFP
  • Tag / Fusion Protein
    • NLS-tdMCP-eGFP (C terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTol-Promoter:NLS-tdMCP-eGFP was a gift from Florence Marlow (Addgene plasmid # 86248 ; http://n2t.net/addgene:86248 ; RRID:Addgene_86248)
  • For your References section:

    Dynamic visualization of transcription and RNA subcellular localization in zebrafish. Campbell PD, Chao JA, Singer RH, Marlow FL. Development. 2015 Apr 1;142(7):1368-74. doi: 10.1242/dev.118968. Epub 2015 Mar 10. 10.1242/dev.118968 PubMed 25758462