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pSJ1458 (pFA6-link-mTurquoise2-CaURA3MX)
(Plasmid #86424)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 86424 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pFA6-link-CaURA3MX
  • Backbone size w/o insert (bp) 4200
  • Total vector size (bp) 4900
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mTurquoise2
  • Species
    S. cerevisiae (budding yeast), Synthetic; yeast codon optimized mTurquoise2 for expression in S. cerevisiae
  • Insert Size (bp)
    716

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AscI (not destroyed)
  • 3′ cloning site PacI (not destroyed)
  • 5′ sequencing primer F5
  • 3′ sequencing primer R3
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    cloned into pKT209, a vector from Kurt Thorn
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

use F5/R3 primers

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSJ1458 (pFA6-link-mTurquoise2-CaURA3MX) was a gift from Sue Jaspersen (Addgene plasmid # 86424 ; http://n2t.net/addgene:86424 ; RRID:Addgene_86424)
  • For your References section:

    Structured illumination with particle averaging reveals novel roles for yeast centrosome components during duplication. Burns S, Avena JS, Unruh JR, Yu Z, Smith SE, Slaughter BD, Winey M, Jaspersen SL. Elife. 2015 Sep 15;4. doi: 10.7554/eLife.08586. 10.7554/eLife.08586 PubMed 26371506