mouse pGL3-ank3_1-promoter firefly luciferase
(Plasmid
#86436)
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PurposeMouse pGL3-ank3_1-promoter firefly luciferase, ca 2000 base pairs around TSS exon 1e
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Depositing Labs
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Depositing OrganizationRoyal College of Surgeons in Ireland
Located in Ireland -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 86436 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 86436-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 86436-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepGL3
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 4818
- Total vector size (bp) 6696
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Modifications to backboneinsertion of ank3_1 promoter sequence
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameank3_1 promoter
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Alt nameankyrin-G gene promoter 1
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Alt nameMYRT-37 gene promoter 1
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SpeciesM. musculus (mouse)
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Insert Size (bp)1903
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Mutationn/a
- Promoter ankyrin-3_1
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Tag
/ Fusion Protein
- n/a
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (unknown if destroyed)
- 3′ cloning site NheI (unknown if destroyed)
- 5′ sequencing primer RVPrimer3
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
König et al., NF-κB regulates neuronal ankyrin-G via a negative feedback loop, Scientific Reports, 2017
DNA (Catalog # 86436-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 86436-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mouse pGL3-ank3_1-promoter firefly luciferase was a gift from Hans-Georg Koenig & Jochen Prehn (Addgene plasmid # 86436 ; http://n2t.net/addgene:86436 ; RRID:Addgene_86436)
Map uploaded by the depositor.
