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(Plasmid #86470)


Item Catalog # Description Quantity Price (USD)
Plasmid 86470 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *

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  • Vector backbone
  • Backbone manufacturer
  • Modifications to backbone
    added HIS10-TEV-HA (HTH) epitope tag downstream of the GAL1 promoter between the HindIII and KpnI restriction sites
  • Vector type
    Yeast Expression
  • Promoter GAL1
  • Selectable markers
  • Tag / Fusion Protein
    • HIS10-TEV-HA (N terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number

Cloning Information

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYES2-HTH was a gift from Akash Gunjan (Addgene plasmid # 86470 ; ; RRID:Addgene_86470)
  • For your References section:

    Histone levels are regulated by phosphorylation and ubiquitylation-dependent proteolysis. Singh RK, Kabbaj MH, Paik J, Gunjan A. Nat Cell Biol. 2009 Aug;11(8):925-33. doi: 10.1038/ncb1903. Epub 2009 Jul 5. 10.1038/ncb1903 PubMed 19578373