PurposeExpresses chimeric fusion of PH domain of Btk and kinase domain of Akt1
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||86591||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepFastBac Dual
- Total vector size (bp) 1602
Vector typeInsect Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Cloned by Iva LUCIC
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pFBD-Btk(PH)Akt1(KD) was a gift from Thomas Leonard & Ivan Yudushkin (Addgene plasmid # 86591 ; http://n2t.net/addgene:86591 ; RRID:Addgene_86591)
For your References section:PI(3,4,5)P3 Engagement Restricts Akt Activity to Cellular Membranes. Ebner M, Lucic I, Leonard TA, Yudushkin I. Mol Cell. 2017 Feb 2;65(3):416-431.e6. doi: 10.1016/j.molcel.2016.12.028. 10.1016/j.molcel.2016.12.028 PubMed 28157504