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Addgene

p4459 FLAG-hPLIC-1 NTF
(Plasmid #8664)

Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8664 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCS2+
  • Backbone size w/o insert (bp) 4095
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hPLIC-1
  • Alt name
    PLIC-1
  • Species
    H. sapiens (human)
  • Mutation
    N-terminal fragment: amino acids 1-112 of hPLIC-1 fused to peptide CYRFSRVYRH
  • Entrez Gene
    UBQLN1 (a.k.a. DA41, DSK2, PLIC-1, UBQN, XDRP1)
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV-F
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See author's map.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p4459 FLAG-hPLIC-1 NTF was a gift from Peter Howley (Addgene plasmid # 8664 ; http://n2t.net/addgene:8664 ; RRID:Addgene_8664)
  • For your References section:

    The hPLIC proteins may provide a link between the ubiquitination machinery and the proteasome. Kleijnen MF, Shih AH, Zhou P, Kumar S, Soccio RE, Kedersha NL, Gill G, Howley PM. Mol Cell 2000 Aug;6(2):409-19. 10.1016/S1097-2765(00)00040-X PubMed 10983987