pRB326
(Plasmid #8667)

• Depositing Lab: David Botstein
• Publication: Schatz et al Mol Cell Biol 1986 Nov;6(11):3722-33.

Backbone

• Vector backbone: YCp50
• Backbone manufacturer: Kuo et al, Mol Cell Biol. 1983 October; 3(10): 1730
• Vector type: Yeast Expression ; Centromeric
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Unknown
• Growth instructions: Don't know
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: TUB1
• Species: S. cerevisiae (budding yeast)
• Entrez Gene: TUB1 (a.k.a. YML085C)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: See comment (unknown if destroyed)
• 3′ cloning site: See comment (unknown if destroyed)
• 5′ sequencing primer: N/A

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The TUB1 centromere plasmid was constructed by ligating a TUB1 fragment from the SphI site (1.1 kilobases before the start codon) to a BglII site (0.5 kb beyond the stop codon) in place of the small SphI-to-BamHI fragment of YCp50. For more information on YCp50, including map, search for YCp50 in Addgene's vector db.