Purpose(Empty Backbone) Tetracycline inducible (2TO version) expression of guide RNA targeted to AAVS1 locus
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||86697||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerLudovic Vallier
- Backbone size (bp) 9585
Modifications to backboneRemoved H1 TO and gRNA scaffold by digestion with BstBI and HincII. Replaced with a 2TO H1 promoter and gRNA scaffold.
Vector typeMammalian Expression, CRISPR
- Promoter H1 2TO
Growth in Bacteria
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer CGAACGCTGACGTCATCAACC
- 3′ sequencing primer GGGCTATGAACTAATGACCCCG (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAAV-Puro_siKO-2TO was a gift from Ludovic Vallier (Addgene plasmid # 86697 ; http://n2t.net/addgene:86697 ; RRID:Addgene_86697)
For your References section:Optimized inducible shRNA and CRISPR/Cas9 platforms for in vitro studies of human development using hPSCs. Bertero A, Pawlowski M, Ortmann D, Snijders K, Yiangou L, Cardoso de Brito M, Brown S, Bernard WG, Cooper JD, Giacomelli E, Gambardella L, Hannan NR, Iyer D, Sampaziotis F, Serrano F, Zonneveld MC, Sinha S, Kotter M, Vallier L. Development. 2016 Dec 1;143(23):4405-4418. 10.1242/dev.138081 PubMed 27899508