Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

Optimized inducible shRNA and CRISPR/Cas9 platforms for in vitro studies of human development using hPSCs.
Bertero A, Pawlowski M, Ortmann D, Snijders K, Yiangou L, Cardoso de Brito M, Brown S, Bernard WG, Cooper JD, Giacomelli E, Gambardella L, Hannan NR, Iyer D, Sampaziotis F, Serrano F, Zonneveld MC, Sinha S, Kotter M, Vallier L
Development. 2016 Dec 1;143(23):4405-4418.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
86695pAAV-Puro_siKDTetracycline inducible expression of shRNA targeted to AAVS1 locus
Loading...
86696pAAV-Puro_siKOTetracycline inducible expression of guide RNA targeted to AAVS1 locus
Loading...
86697pAAV-Puro_siKO-2TOTetracycline inducible (2TO version) expression of guide RNA targeted to AAVS1 locus
Loading...
86698pAAV-Neo_CAG-Cas9Cas9 expression vector for targeting to the AAVS1 locus
Loading...