PurposeTissue-specific FLP expression plasmid for C. elegans
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||86730||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector typeWorm Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert namePunc-47::FLP D5
SpeciesC. elegans (nematode)
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBN303 was a gift from Peter Askjaer (Addgene plasmid # 86730 ; http://n2t.net/addgene:86730 ; RRID:Addgene_86730)
For your References section:An Efficient FLP-Based Toolkit for Spatiotemporal Control of Gene Expression in Caenorhabditis elegans. Munoz-Jimenez C, Ayuso C, Dobrzynska A, Torres-Mendez A, Ruiz PC, Askjaer P. Genetics. 2017 Aug;206(4):1763-1778. doi: 10.1534/genetics.117.201012. Epub 2017 Jun 23. 10.1534/genetics.117.201012 PubMed 28646043