pET16b-Antp
(Plasmid
#86866)
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PurposeBacterial expression of fusion protein with cell penetrating peptide, antennapedia fragment, at C-terminus.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 86866 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepET16b
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5711
- Total vector size (bp) 5781
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLinker-Antp
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Alt nameAntp cell penetrating peptide
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SpeciesD. melanogaster (fly)
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Insert Size (bp)69
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Tag
/ Fusion Protein
- Antp (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site BamHi (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7 termination (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET16b-Antp was a gift from Michael Chin (Addgene plasmid # 86866 ; http://n2t.net/addgene:86866 ; RRID:Addgene_86866) -
For your References section:
Genomic DNA recombination with cell-penetrating peptide-tagged cre protein in mouse skeletal and cardiac muscle. Chien WM, Liu Y, Chin MT. Genesis. 2014 Jul;52(7):695-701. doi: 10.1002/dvg.22782. Epub 2014 May 5. 10.1002/dvg.22782 PubMed 24753043
Map uploaded by the depositor.
