6xHis-TEV-SMO-1(GG)
(Plasmid #87054)

• Purpose: Expresses 6xHis-tagged smo-1(GG), with a TEV protease cleavage site between the tag and the ORF
• Depositing Lab: Ronald Hay
• Publication: Pelisch et al Nat Commun. 2014 Dec 5;5:5485. doi: 10.1038/ncomms6485.

Backbone

• Vector backbone: pHISTEV30a
• Backbone size w/o insert (bp): 5365
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: smo-1
• Alt name: C. elegans SUMO
• Species: C. elegans (nematode)
• Mutation: The last aa was removed to render processed SMO-1 ready for conjugation
• GenBank ID: NM_058441.4
• Entrez Gene: smo-1 (a.k.a. CELE_K12C11.2)
• Promoter: T7
• Tag / Fusion Protein:
  • 6xHis-TEV (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (unknown if destroyed)
• 3′ cloning site: NotI (unknown if destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7term

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry