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pKT0175
(Plasmid #8733)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 8733 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pFA6a-link
  • Backbone size w/o insert (bp) 4900
  • Vector type
    Yeast Expression
  • Selectable markers
    C. albicans URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    yECitrine
  • Alt name
    Codon-optimized GFP
  • Mutation
    yEGFP with S65G, V68L, Q69M, S72A, T203Y
  • Tag / Fusion Protein
    • Codon optimized linker (N terminal on backbone)

Cloning Information

Resource Information

Depositor Comments

Full name of this plasmid is pFA6a-link-yECitrine-CaURA3. yECitrine is created by mutagenesis from a codon-optimized green
fluorescent protein - yEGFP1 (Cormack et al. 1997). This yEGFP
variant is cloned into pDH5, replacing YFP. An codon-optimized linker
was added into the yEGFP variant to improve expression level. C.
albicans URA3 (CaURA3) was introduced by subcloning the BglII/SacI
fragment of pAG60 (Goldstein et al., 1999) into the plasmid, in place
of SpHIS5.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKT0175 was a gift from Kurt Thorn (Addgene plasmid # 8733)
  • For your References section:

    Optimized cassettes for fluorescent protein tagging in Saccharomyces cerevisiae. Sheff MA, Thorn KS. Yeast 2004 Jun;21(8):661-70. 10.1002/yea.1130 PubMed 15197731