|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||8732||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4894
Vector typeYeast Expression
Growth in Bacteria
Alt nameCodon-optimized GFP
MutationyEGFP with S65G, V68L, Q69M, S72A, T203Y
/ Fusion Protein
- Codon optimized linker (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ sequencing primer N/A (Common Sequencing Primers)
Terms and Licenses
Full name of this plasmid is pFA6a-link-yECitrine-KanR. yECitrine is created by mutagenesis from a codon-optimized green
fluorescent protein - yEGFP1 (Cormack et al. 1997). This yEGFP
variant is cloned into pDH5, replacing YFP. An codon-optimized linker
was added into the yEGFP variant to improve expression level. The
G418 resistance marker (KanR) was introduced into the plasmid by
subcloning the BglII/EcoRI fragment of pDH3 (Hailey et al., 2002) into
the plasmid, in place of SpHIS5.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pKT0140 was a gift from Kurt Thorn (Addgene plasmid # 8732)
For your References section:Optimized cassettes for fluorescent protein tagging in Saccharomyces cerevisiae. Sheff MA, Thorn KS. Yeast 2004 Jun;21(8):661-70. 10.1002/yea.1130 PubMed 15197731