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pKT0149
(Plasmid #8740)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 8740 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pFA6a-link
  • Backbone size w/o insert (bp) 4738
  • Vector type
    Yeast Expression
  • Selectable markers
    S. pombe HIS5

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    yESapphire
  • Alt name
    Codon-optimized GFP
  • Mutation
    yEGFP with S72A, Y145F, T203I
  • Tag / Fusion Protein
    • Codon optimized linker (N terminal on backbone)

Cloning Information

Resource Information

Depositor Comments

Full name of this plasmid is pFA6a-link-yESapphire-SpHIS5. yESapphire is created by mutagenesis from a codon-optimized green
fluorescent protein - yEGFP1 (Cormack et al. 1997). This yEGFP
variant is cloned into pDH5, replacing YFP. An codon-optimized linker
was added into the yEGFP variant to improve expression level. The
selectable marker, Sz. pombe HIS5 (SpHIS5), complements S. cerevisiae
HIS3.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKT0149 was a gift from Kurt Thorn (Addgene plasmid # 8740)
  • For your References section:

    Optimized cassettes for fluorescent protein tagging in Saccharomyces cerevisiae. Sheff MA, Thorn KS. Yeast 2004 Jun;21(8):661-70. 10.1002/yea.1130 PubMed 15197731