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pKT0146
(Plasmid #8742)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 8742 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pFA6a-link
  • Backbone size w/o insert (bp) 5416
  • Vector type
    Yeast Expression
  • Selectable markers
    S. pombe HIS5

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    tdimer2
  • Tag / Fusion Protein
    • Codon optimized linker (N terminal on backbone)

Cloning Information

Resource Information

Depositor Comments

Full name of this plasmid is pFA6a-link-tdimer2-SpHIS5. tdimer2 is described in Campbell et al., 2002.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKT0146 was a gift from Kurt Thorn (Addgene plasmid # 8742)
  • For your References section:

    Optimized cassettes for fluorescent protein tagging in Saccharomyces cerevisiae. Sheff MA, Thorn KS. Yeast 2004 Jun;21(8):661-70. 10.1002/yea.1130 PubMed 15197731