3269 pBS Bcl-2
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||8764||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector backbonepBluescript KS
- Backbone size w/o insert (bp) 3000
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Strain(s)XL1 Blue
Copy numberHigh Copy
SpeciesM. musculus (mouse)
Insert Size (bp)600
Entrez GeneBcl2 (a.k.a. Bcl-2, C430015F12Rik, D630044D05Rik, D830018M01Rik)
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer SP6
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Main use: in situ probe. Cut with HindIII and use T7 polymerase for antisense probe. Cut with XbaI and use T3 for sense probe.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:3269 pBS Bcl-2 was a gift from Stanley Korsmeyer (Addgene plasmid # 8764 ; http://n2t.net/addgene:8764 ; RRID:Addgene_8764)
For your References section:Meiotic recombination between yeast artificial chromosomes yields a single clone containing the entire BCL2 protooncogene. Silverman GA, Green ED, Young RL, Jockel JI, Domer PH, Korsmeyer SJ. Proc Natl Acad Sci U S A 1990 Dec;87(24):9913-7. 10.1073/pnas.87.24.9913 PubMed 2263642