3349 pSFFV-neo Bcl-2
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||8769||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 7200
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Strain(s)XL1 Blue
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)720
Entrez GeneBCL2 (a.k.a. Bcl-2, PPP1R50)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer SFFV-F (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
ORF contains SacI and BamHI sites.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:3349 pSFFV-neo Bcl-2 was a gift from Stanley Korsmeyer (Addgene plasmid # 8769 ; http://n2t.net/addgene:8769 ; RRID:Addgene_8769)
For your References section:BCL-2 is phosphorylated and inactivated by an ASK1/Jun N-terminal protein kinase pathway normally activated at G(2)/M. Yamamoto K, Ichijo H, Korsmeyer SJ. Mol Cell Biol 1999 Dec;19(12):8469-78. PubMed 10567572
Map uploaded by the depositor.