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3396 pBM272 Bax
(Plasmid #8770)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 8770 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBM272
  • Backbone size w/o insert (bp) 8259
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Bax
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    600
  • GenBank ID
    NM_007527
  • Entrez Gene
    Bax

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer Gal10pro-F
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

Murine BAX downstream of GAL10 promoter, also contains yeast URA3 gene, sequences representing CEN4 and ARS1.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    3396 pBM272 Bax was a gift from Stanley Korsmeyer (Addgene plasmid # 8770 ; http://n2t.net/addgene:8770 ; RRID:Addgene_8770)
  • For your References section:

    Biochemical and genetic analysis of the mitochondrial response of yeast to BAX and BCL-X(L). Gross A, Pilcher K, Blachly-Dyson E, Basso E, Jockel J, Bassik MC, Korsmeyer SJ, Forte M. Mol Cell Biol 2000 May;20(9):3125-36. 10.1128/MCB.20.9.3125-3136.2000 PubMed 10757797