Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

3396 pBM272 Bax
(Plasmid #8770)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 8770 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBM272
  • Backbone size w/o insert (bp) 8259
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Bax
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    600
  • GenBank ID
    NM_007527
  • Entrez Gene
    Bax

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer Gal10pro-F
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Murine BAX downstream of GAL10 promoter, also contains yeast URA3 gene, sequences representing CEN4 and ARS1.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    3396 pBM272 Bax was a gift from Stanley Korsmeyer (Addgene plasmid # 8770 ; http://n2t.net/addgene:8770 ; RRID:Addgene_8770)
  • For your References section:

    Biochemical and genetic analysis of the mitochondrial response of yeast to BAX and BCL-X(L). Gross A, Pilcher K, Blachly-Dyson E, Basso E, Jockel J, Bassik MC, Korsmeyer SJ, Forte M. Mol Cell Biol 2000 May;20(9):3125-36. 10.1128/MCB.20.9.3125-3136.2000 PubMed 10757797